海水养殖真鲷弧菌病病原菌外毒素的分离、纯化及生物学活性   总被引：18，自引：2，他引：18  

吴后波  潘金培 《海洋与湖沼》2002,33(1):83-89

1996年6月初，对福建省平潭县真鲷网箱养殖场发生的弧菌病进行研究，并确定该病病原菌为最小弧菌（Vibrio mimicus）。采用硫酸铵沉淀、DEAE－纤维素层析及Sephadex-G100层析等方法对最小弧菌产生的外毒素进行研究。结果表明，该外毒素为单一的多肽，分子量为30．456kD，对真鲷的LD50为4．48μg，对真鲷等养殖动物的溶血性最强，对Vero细胞的CD50值为0．48μg，肠毒性弱阳性。该外毒素与最小弧菌产生的其他毒力因子相比，性质独特，是一类新的毒力因子，建议命名为Vm-Pm毒素。  相似文献   

Spring and summer phytoplankton communities in the Chukchi and Eastern Beaufort Seas   总被引：2，自引：0，他引：2  

Victoria Hill  Glenn Cota  Dean Stockwell 《Deep Sea Research Part II: Topical Studies in Oceanography》2005,52(24-26):3369

Phytoplankton pigments and size-fractionated biomass in the Chukchi and Beaufort Seas showed spatial and temporal variation during the spring and summer of 2002. Cluster analysis of pigment ratios revealed different assemblages over the shelf, slope and basin regions. In spring, phytoplankton with particle sizes greater than 5 μm, identified as diatoms and/or haptophytes, dominated over the shelf. Smaller (<5 μm) phytoplankton containing chlorophyll b, most likely prasinophytes, were more abundant over the slope and basin. Due to extensive ice cover at this time, phytoplankton experienced low irradiance, but nutrients were near maximal for the year. By summer, small prasinophytes and larger haptophytes and diatoms co-dominated in near-surface assemblages in largely ice-free waters when nitrate was mostly depleted. Deeper in the water column at 1–15% of the surface irradiance larger sized diatoms were still abundant in the upper nutricline. Phytoplankton from the shelf appeared to be advected through Barrow Canyon to the adjacent basin, explaining similar composition between the two areas in spring and summer. Off-shelf advection was much less pronounced for other slope and basin areas, which are influenced by the low-nutrient Beaufort gyre circulation, leading to a dominance of smaller prasinophytes and chlorophytes. The correlation of large-sized fucoxanthin containing phytoplankton with the higher primary production measurements shows promise for trophic status to be estimated using accessory pigment ratios.  相似文献   

Atlas of Japan (East) Sea hydrographic properties in summer, 1999     

Lynne D. Talley  Pavel Tishchenko  Vladimir Luchin  Alexander Nedashkovskiy  Sergey Sagalaev  Dong-Jin Kang  Mark Warner  Dong-Ha Min 《Progress in Oceanography》2004,61(2-4):277

Hydrographic properties from CTD and discrete bottle sample profiles covering the Japan (East) Sea in summer, 1999, are presented in vertical sections, maps at standard depths, maps on isopycnal surfaces, and as property–property distributions. This data set covers most of the Sea with the exception of the western boundary region and northern Tatar Strait, and includes nutrients, pH, alkalinity, and chlorofluorocarbons, as well as the usual temperature, salinity, and oxygen observations.  相似文献   

Flow fields in reflected waves at a sloped sea wall     

Gerald Müller   《Ocean Engineering》2007,34(11-12):1786-1789

Wave run-up, and flow visualization experiments were conducted with a 1:2 sloped sea wall model. The visualization experiments gave an overview of flow fields in reflected, non-breaking conditions. Maximum particle velocities were found to be significantly smaller than suggested in the literature. Downrush produced a fast sheet flow, extending down to the toe of the sea wall. This created a ‘reverse’ breaker during the retreat of the initially non-breaking wave, which explains the high-energy dissipation rates for non-breaking waves reported in the literature. Embankments may therefore be exposed to wave impact pressures in areas up to 1.18H₀ below MWL.  相似文献   

Experimental activities that intentionally perturb the marine environment: Implications for the marine environmental protection and marine scientific research provisions of the 1982 United Nations Convention on the Law of the Sea     

Philomène A. Verlaan 《Marine Policy》2007

The global community's ability to assess the environmental effects of marine scientific research (MSR) as required by the 1982 United Nations Convention on the Law of the Sea's (UNCLOS) marine environment provisions (MEP) is limited, as shown by experimental activities that intentionally manipulate the marine environment. Such work enhances knowledge of the ocean, but it may also have significant environmental effects. This growing use of the ocean as a laboratory has global scientific, environmental, legal and policy implications. Examined here is the relationship between the MSR and the MEP provisions of UNCLOS. International, science-driven guidelines are proposed.  相似文献   

Comparison among four kinds of data of sea surface wind stress in the South China Sea   总被引：1，自引：0，他引：1  

Xie Qiang  Wang Weiqiang  Mao Qingwen 《海洋学报(英文版)》2002,21(2):263-273

By using remote sensing (ERS) data, FSU data, COADS data and Hellerman & Rosen-stein objective analysis data to analyze the sea surface wind stress in the South China Sea, it is found that the remote sensing data have higher resolution and more reasonable values. Therefore we suggest that remote sensing data be chosen in the study of climatological features of sea surface wind stress and its seasonal variability in the South China Sea, especially in the study of small and middle scale eddies.  相似文献   

南沙群岛海域水体漫衰减系数Kd(490)的特性研究   总被引：6，自引：1，他引：6  

张清凌  陈楚群  施平 《热带海洋学报》2003,22(1):9-16

对南沙群岛海区水体Kd(490）的特性进行了深入分析和探讨,研究结果表明：Kd（490）的垂直分布与同步测量的海水叶绿素a浓度的垂直分布有很强的相关性;Kd（490）的垂直分布反映出该区域海水存在较强的分层现象,研究中还发现上行辐亮度漫衰减系数KL（490）与Kd（490）垂直变化趋势相当一致,但是以二者达到最大值的深度为界,上层水体的KL（490）值稍大于Kd(490）值,在这一深度以下则相反。  相似文献   

海水温度和盐度对泥蚶幼虫和稚贝生长及存活的影响   总被引：24，自引：0，他引：24  

尤仲杰  徐善良  边平江  陈坚 《海洋学报》2001,23(6):108-113

1996～1997和1999年在浙江省乐清市东发水产育苗场和福建省宁德市四海水产育苗场分别研究了海水温度和盐度对泥蚶浮游幼虫和稚贝生长及存活的影响,结果表明,泥蚶浮游幼虫的适宜温度为25～33℃,最适生长温度为28～30℃.稚贝的适宜温度为15～35℃,最适生长温度为25～30℃.浮游幼虫的适宜盐度为16.54～30.02,最适生长盐度为1654～23.38;稚贝的适宜盐度为10.01～30.02,最适生长盐度为10.01～23.38.泥蚶幼虫和稚贝对高温和低盐海水有较强的适应能力,这与它们夏季的繁殖期和自然分布于内湾河口区是相适应的.  相似文献   

南海晚第四纪硅藻遗体及其地质意义Ⅵ.硅藻的2个新种和7个新记录     

程兆第  蓝东兆 《台湾海峡》1995,14(1):40-43

本文记述了南海晚第四纪沉积物中发现的两个新种[线形辐环藻（Actinocyclus lineatus Liu,Cheng et Lan sp.nov.）、星圆筛藻（Coscinodiscus aster-aeus Cheng,Liu et Lan sp.nov.）]以及在我国首次记录的圆筛藻的7个种。  相似文献   

Active spreading and hydrothermalism in North Fiji Basin (SW Pacific). Results of Japanese French Cruise Kaiyo 87     

Jean-Marie Auzende  Eiichi Honza  Xavier Boespflug  Satendra Deo  Jean-Philippe Eissen  Jun Hashimoto  Philippe Huchon  Junichiro Ishibashi  Yo Iwabuchi  Philip Jarvis  Masato Joshima  Kiyoyuki Kisimoto  Yasuto Kuwahara  Yves Lafoy  Tsuyoshi Matsumoto  Jean-Pierre Maze  Kiyohiko Mitsuzawa  Hiroyasu Monma  Takeshi Naganuma  Yukihiro Nojiri  Suguru Ohta  Kiyoshi Otsuka  Yoshihisa Okuda  Hélène Ondreas  Akira Otsuki  Etienne Ruellan  Myriam Sibuet  Manabu Tanahashi  Takeo Tanaka  Tetsuro Urabe 《Marine Geophysical Researches》1990,12(4):269-283

The aim of the Japanese-French Kaiyo 87 cruise was the study of the spreading axis in the North Fiji Basin (SW Pacific). A Seabeam and geophysical survey allowed us to define the detailed structure of the active NS spreading axis between 16° and 22° S and its relationships with the left lateral motion of the North Fiji Fracture Zone. Between 21° S and 18°10′ S, the spreading axis trends NS. From 18°10 S to 16°40 S the orientation of the spreading axis changes from NS to 015°. North of 16°40′ S the spreading axis trends 160°. These two 015° and 160° branches converge with the left lateral North Fiji fracture zone around 16°40′ S to define an RRFZ triple junction. Water sampling, dredging and photo TV deep towing give new information concerning the hydrothermal activity along the spreading axis. The discovery of hydrothermal deposits associated with living communities confirms this activity.  相似文献